# Simple example summary spreadsheet for tab2mage.pl script. # This file illustrates how channels should be combined when describing a two-color hybridization (e.g. Cy3/Cy5). # Lines starting with a '#' are treated as comments and are ignored. Experiment section domain ebi.ac.uk accession E-EXML-1 quality_control dye_swap_quality_control experiment_design_type strain_or_line_design;co-expression_design name Transcriptome analysis of invasive verses non-invasive strains of budding yeast description An experiment was performed to... release_date 2004-08-30 submission_date 2004-07-28 submitter John Falstaff submitter_email jfalstaff@wagglespike.com investigator Bill Shakespeare investigator_email bills@wagglespike.com organization Windsor Laboratories address Windsor, Ontario, Canada # The publication details below are fictitious publication_title Comparison of the transcriptomes of invasive verses non-invasive strains of budding yeast authors John Falstaff; Robin Goodfellow; Bill Shakespeare journal Nature Genetics volume 12 issue 4 pages 123-456 year 2004 pubmed_id 12345678 # Protocol text may be formatted using standard HTML tags Protocol section accession text name P-EXML-1 Cells were grown in YPD (1% yeast extract/2% peptone/2% glucose) to an OD600 of approximately 0.8 Yeast growth P-EXML-2 Your protocol text here Yeast cell harvesting P-EXML-3 Your protocol text here Cell lysis and RNA prep P-EXML-4 Your protocol text here cDNA labeling P-EXML-5 Your protocol text here Hybridization P-EXML-6 Your protocol text here Scanning Hybridization section File[raw] Array[accession] Array[serial] Protocol[grow] Protocol[treatment] Protocol[extraction] Protocol[labeling] Protocol[hybridization] Protocol[scanning] BioSource Sample Extract LabeledExtract Hybridization BioSourceMaterial SampleMaterial ExtractMaterial LabeledExtractMaterial Dye FactorValue[StrainOrLine] BioMaterialCharacteristics[Organism] BioMaterialCharacteristics[StrainOrLine] BioMaterialCharacteristics[Sex] Data1.txt A-EXML-1 244532 P-EXML-1 P-EXML-2 P-EXML-3 P-EXML-4 P-EXML-5 P-EXML-6 S288C S288C S288C S288C Cy5 Dye Swap 1 whole_organism whole_organism total_RNA synthetic_DNA Cy5 S288C Saccharomyces cerevisiae S288C mating_type_a Data1.txt A-EXML-1 244532 P-EXML-1 P-EXML-2 P-EXML-3 P-EXML-4 P-EXML-5 P-EXML-6 Sigma1278b Sigma1278b Sigma1278b Sigma1278b Cy3 Dye Swap 1 whole_organism whole_organism total_RNA synthetic_DNA Cy3 Sigma1278b Saccharomyces cerevisiae Sigma1278b mating_type_a Data2.txt A-EXML-1 244533 P-EXML-1 P-EXML-2 P-EXML-3 P-EXML-4 P-EXML-5 P-EXML-6 S288C S288C S288C S288C Cy3 Dye Swap 2 whole_organism whole_organism total_RNA synthetic_DNA Cy3 S288C Saccharomyces cerevisiae S288C mating_type_a Data2.txt A-EXML-1 244533 P-EXML-1 P-EXML-2 P-EXML-3 P-EXML-4 P-EXML-5 P-EXML-6 Sigma1278b Sigma1278b Sigma1278b Sigma1278b Cy5 Dye Swap 2 whole_organism whole_organism total_RNA synthetic_DNA Cy5 Sigma1278b Saccharomyces cerevisiae Sigma1278b mating_type_a