"Investigation Title" "Solexa analysis of Galapagos finches" "Experiment Description" "Solexa analysis of Galapagos finches" "Experimental Design" "growth_condition_design" "replicate_design" "co-expression_design" "Experimental Factor Name" "STRAINORLINE" "Experimental Factor Type" "strain_or_line" "Experimental Factor Term Source REF" "MGED Ontology" "Quality Control Type" "biological_replicate" "Public Release Date" "2008-01-01" "Person Last Name" "Darwin" "Person First Name" "Charles" "Person Mid Initials" "Person Email" "Charles@darwin.com" "Person Phone" "Person Address" "Beagle Institute of Theoretical Experiments, Down House, Downe, Kent, UK" "Person Affiliation" "Beagle Institute of Theoretical Experiments" "Person Roles" "submitter" "PubMed ID" "Publication Author List" "Darwin, C" "Publication Title" "Solexa analysis of Galapagos finches" "Publication Status" "Submitted" "Protocol Name" "EXTRACTION" "NORMALIZATION" "SOLEXA SEQ" "SOLEXA DATA ACQUISITION" "Protocol Type" "nucleic_acid_extraction" "bioassay_data_transformation" "Protocol Description" "All cDNA samples were prepared by treating ~1 mg of total RNA for 30 min with amplification grade RNase-free DNase (Invitrogen), according to the manufacturer's protocols. PolyA enriched RNA was then prepared using an oligo(dT) selection kit (Oligotex Direct mRNA MiniKit, Qiagen). The resulting poly(A)-enriched RNA was then converted to double-stranded cDNA using a cDNA synthesis kit (Superscript choice system for cDNA synthesis, Invitrogen)." "FASTQ files of sequencing reads were retrieved from the sequencing machine and converted into FASTA files. FASTA sequences were filtered, aligned to the genome and relative gene expression scores calculated based on a finch reference genome." "Protocol Term Source REF" "MGED Ontology" "Term Source Name" "MGED Ontology" "Term Source File" "http://mged.sourceforge.net/ontologies/MGEDontology.php" "Term Source Version" "1.3.0.1" "SDRF File" "solexa_example.sdrf.txt"